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doctoral thesis
UČINAK PENTADEKAPEPTIDA BPC 157 NA APIKALNI PARODONTITIS UZROKOVAN EKSPOZICIJOM PULPE ZUBA U ŠTAKORA
2023. urn:nbn:hr:236:148431
Juzbašić, Martina
Josip Juraj Strossmayer University of Osijek
Doctoral studies

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Juzbašić, M. (2023). UČINAK PENTADEKAPEPTIDA BPC 157 NA APIKALNI PARODONTITIS UZROKOVAN EKSPOZICIJOM PULPE ZUBA U ŠTAKORA (Doctoral thesis). Osjek: Josip Juraj Strossmayer University of Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:236:148431

Juzbašić, Martina. "UČINAK PENTADEKAPEPTIDA BPC 157 NA APIKALNI PARODONTITIS UZROKOVAN EKSPOZICIJOM PULPE ZUBA U ŠTAKORA." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, 2023. https://urn.nsk.hr/urn:nbn:hr:236:148431

Juzbašić, Martina. "UČINAK PENTADEKAPEPTIDA BPC 157 NA APIKALNI PARODONTITIS UZROKOVAN EKSPOZICIJOM PULPE ZUBA U ŠTAKORA." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, 2023. https://urn.nsk.hr/urn:nbn:hr:236:148431

Juzbašić, M. (2023). 'UČINAK PENTADEKAPEPTIDA BPC 157 NA APIKALNI PARODONTITIS UZROKOVAN EKSPOZICIJOM PULPE ZUBA U ŠTAKORA', Doctoral thesis, Josip Juraj Strossmayer University of Osijek, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:236:148431

Juzbašić M. UČINAK PENTADEKAPEPTIDA BPC 157 NA APIKALNI PARODONTITIS UZROKOVAN EKSPOZICIJOM PULPE ZUBA U ŠTAKORA [Doctoral thesis]. Osjek: Josip Juraj Strossmayer University of Osijek; 2023 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:236:148431

M. Juzbašić, "UČINAK PENTADEKAPEPTIDA BPC 157 NA APIKALNI PARODONTITIS UZROKOVAN EKSPOZICIJOM PULPE ZUBA U ŠTAKORA", Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Osjek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:236:148431

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TitleUČINAK PENTADEKAPEPTIDA BPC 157 NA APIKALNI PARODONTITIS UZROKOVAN EKSPOZICIJOM PULPE ZUBA U ŠTAKORA
Title (english)EFFECT OF PENTADECAPEPTIDE BPC 157 ON APICAL PERIODONTITIS CAUSED BY PULP EXPOSURE IN RATS
AuthorMartina Juzbašić
MentorPredrag Sikirić (mentor)
Committee memberMartina Smolić (predsjednik povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
(Doctoral studies)
Osjek
Defense date and country2023-10-11, Croatia
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Universal decimal classification
(UDC)		60 - Biotechnology. Genetic engineering. Products of cloning
Abstract
Apikalni parodontitis jedno je od najčešćih upalnih stanja zuba i široko je rasprostranjen. Unatoč nerijetkom neuspjehu mehaničkog čišćenja kao zlatnog standarda i dalje ne postoji alternativna terapija koja bi utjecala na smanjenje oštećenja uzrokovanoga otvaranjem pulpe te time sačuvala zub. Pentadekapeptid BPC 157 u raznim dosadašnjim životinjskim eksperimentalnim modelima pokazao je velik potencijal cijeljenja različitih tkiva. Cilj je ovog istraživanja pokazati učinak pentadekapeptida BPC 157 na modelu apikalnog parodontitisa uzrokovanog ekspozicijom pulpe zuba kod štakora. Tretirane grupe štakora primale su BPC 157 per os u dozi od 10ng/kg, odnosno 10μg/kg otopljen u vodi za piće, kontrolna grupa je cijelo vrijeme pokusa pila vodu. Na unaprijed određenim intervalima štakorima su uzeti uzorci sline i brisevi usne šupljine za mikrobiološku analizu te je učinjena hemisekcija maksile s ciljem uzimanja oštećenog zuba i okolnog tkiva za histološku i molekularnu analizu. Cijeli pokus praćen je makroskopski i radiološki. Makroskopsko promatranje pokazalo je da je preostali koronarni dio zubnog tkiva oko trepanacijskog otvora skloniji pucanju u kontrolnoj grupi štakora. Mikrobiološka analiza pokazala je jednak broj kolonija i bakterijskih vrsta kod svih štakora. Područje radiografskog gubitka periapikalne kosti i defekta u furkaciji korijena bilo je znatno manje kod štakora koji su uzimali BPC 157. Histološka analiza pokazala je pojačanu razinu inflamatornog i staničnog uzorka i kod tretiranih i kontrolnih grupa, dok je statistički značajna razlika pronađena u broju krvožilnih prostora kojih je puno više kod tretiranih BPC-om 157. Rezultati RT-qPCR analize gena na razini mRNA, gdje su ispitani uzorci tretiranih grupa štakora BPC-om 157 i uspoređeni s kontrolnom grupom, pokazali su razinu ekspresije mRNA za gene Egr1 i Ptgs2 <1, a za gene Nos2 i Nos3 >1. Ovaj pozitivan učinak BPC-a 157 otvara mogućnosti za daljnja istraživanja i novi pristup liječenju apikalnog parodontitisa.
Abstract (english)
Apical periodontitis is one of the most common inflammatory conditions of the teeth. Despite the frequent failure of mechanical cleaning as the gold standard, there is still no alternative therapy that would reduce the damage caused by the opening of the pulp and preserve the tooth. The pentadecapeptide BPC 157 has shown great healing potential in various animal studies in many experimental models to date. This study aimed to analyze the effect of pentadecapeptide BPC 157 on a model of apical periodontitis. The treated groups in the therapy received BPC 157 (10ng/kg or 10μg/kg) per os, while the control group drank water during the experiment. Saliva samples and swabs of the oral cavity were collected for microbiological analysis. The damaged tooth and the surrounding tissue were analyzed histologically and molecularly, while the whole experiment was monitored macroscopically and radiologically.Macroscopic observation showed that the remaining coronal part of the tooth tissue around the trepanation opening is more prone to cracking in the non-treated group. Microbiological analysis showed the same count of colonies and bacterial species in all rats. The area of radiographic periapical bone loss and furcation defect was significantly smaller in rats that were taken BPC 157. Histological analysis showed increased levels of inflammatory and cellular patterns in both treated and untreated animals, while a statistically significant difference was found in the number of blood vessels, which was significantly greater in the rat groups treated with BPC 157. Results of RT -qPCR analysis of genes at the mRNA level, in which samples from rat groups treated with BPC 157 were examined and compared with the control group, showed that pentadecapeptide BPC 157 had a significant effect on the expression of the genes of interest. The mRNA expression level for the Egr1 and Ptgs2 genes was < 1 and for the Nos2 and Nos3 genes > 1. This positive effect of BPC 157 opens up opportunities for further research and a novel approach to apical periodontitis treatment.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:236:148431
Promotion2023
Study programmeTitle: University Postgraduate Interdisciplinary Doctoral Study Molecular Biosciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-11-17 10:19:51