Discrimination of persons belonging to national minorities in the field of work and employment is an obstacle to the realization of their guaranteed economic and social rights. According to the Office of Ombudswoman, ethnic or national affiliation is the most common basis of discrimination, and the field of work and employment is the most common area of discrimination in the Republic of Croatia. Therefore, the aim of this dissertation is to investigate discrimination of persons belonging to national minorities in the field of work and employment in Croatia, as well as existence of multiple discrimination against persons belonging to national minorities and respondents’ knowledge of the legislative framework of protection against discrimination in the Republic of Croatia. A survey of discrimination in the field of work and employment in Croatia was conducted in 2021 on a sample of 520 respondents belonging to national minorities. The parameters of descriptive statistics based on adequate central tendency measures and dispersion measures were determined for data analysis. A chi-square test was done in order to analyze the data in relation to the socio-demographic characteristics of the respondents. The reliability of the measurement scale on the occurrence of discrimination of persons belonging to national minorities in the field of work and employment was determined, the basic segments of employment that contribute to the occurrence of discrimination have been identified as well as the strength of the connection between the attitudes of the respondents belonging to national minorities. The results of the research show the insufficient knowledge of the legislative framework for protection against discrimination in Croatia. The results show that discrimination of minority population is strongly present in the field of work and employment in Croatia and that occurrence of discrimination in certain segments of the employment increase the chance that discrimination will recur or last for a longer period of time. The conducted research is the first quantitative scientific research on the topic of discrimination in the field of work and employment in Croatia on the minority population and represents a quality basis for deeper scientific analysis and research on the topic.